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reporter plasmids prl-tk renilla luciferase  (Promega)

 
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    Promega reporter plasmids prl-tk renilla luciferase
    PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly <t>luciferase</t> gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: <t>Renilla</t> luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.
    Reporter Plasmids Prl Tk Renilla Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reporter plasmids prl-tk renilla luciferase/product/Promega
    Average 90 stars, based on 1 article reviews
    reporter plasmids prl-tk renilla luciferase - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "PARP1-NFATc1-PD1 pathway of maturation and stability of CD8 + T cells is beneficial against chronic Trypanosoma cruzi infection"

    Article Title: PARP1-NFATc1-PD1 pathway of maturation and stability of CD8 + T cells is beneficial against chronic Trypanosoma cruzi infection

    Journal: iScience

    doi: 10.1016/j.isci.2025.112926

    PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly luciferase gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: Renilla luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.
    Figure Legend Snippet: PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly luciferase gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: Renilla luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.

    Techniques Used: Activity Assay, Infection, Transfection, Plasmid Preparation, Luciferase, Control, Incubation, Derivative Assay, MANN-WHITNEY



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    PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly <t>luciferase</t> gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: <t>Renilla</t> luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.
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    Average 90 stars, based on 1 article reviews
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    prl-tk plasmid  (Promega)
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    Promega prl-tk plasmid
    PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly <t>luciferase</t> gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: <t>Renilla</t> luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.
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    https://www.bioz.com/result/prl-tk plasmid/product/Promega
    Average 90 stars, based on 1 article reviews
    prl-tk plasmid - by Bioz Stars, 2026-04
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    Image Search Results


    PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly luciferase gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: Renilla luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.

    Journal: iScience

    Article Title: PARP1-NFATc1-PD1 pathway of maturation and stability of CD8 + T cells is beneficial against chronic Trypanosoma cruzi infection

    doi: 10.1016/j.isci.2025.112926

    Figure Lengend Snippet: PARP1 regulates NFATC1 activity at Pdcd1 promoter in CD8 + T cells responding to T. cruzi infection (A) EL4 T lymphoblast cells were transfected with pGL4.10 reporter plasmid consisting of Pdcd1 promoter region peak1, peak2, or peak3 upstream of firefly luciferase gene (experimental control: pGL4.10 encoding negative peak, transfection efficiency control: Renilla luciferase). NR-9456 Mφ were overlaid with transfected EL4 cells and co-incubated for 24 h in presence or absence of Tc and Olaparib (inhibits PARP1). (B–E) Relative luciferase units (normalized to Renilla luciferase). Individual data point and mean ± SEM values derived from four biological replicates (duplicate readings each) per treatment are plotted. Significance (∗ p value ≤0.05) comparing two groups was analyzed by Student’s unpaired t test or Mann-Whitney U test and identified by a horizontal bar.

    Article Snippet: The pGL4.10[luc2] (Firefly luciferase) and pRL-TK (Renilla luciferase) reporter plasmids were purchased from Promega (Madison, WI).

    Techniques: Activity Assay, Infection, Transfection, Plasmid Preparation, Luciferase, Control, Incubation, Derivative Assay, MANN-WHITNEY